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KMID : 0380620170490050524
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Korean Journal of Food Science and Technology
2017 Volume.49 No. 5 p.524 ~ p.531
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Protective effect on neuronal cells of Orostachys japonicus A. Berger extract against reactive oxygen species-induced neuronal cytotoxicity and active compounds
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Park Su-Bin
Lee Doo-Sang
Kang Jin-Yong
Kim Jong-Min
Park Seon-Kyeong
Kang Jeong-Eun
Kwon Bong-Seok
Park Sang-Hyun
Lee Chang-Jun
Lee Uk
Heo Ho-Jin
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Abstract
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The study aimed to investigate the antioxidant activity and neuroprotective effect of the ethyl acetate fraction from Orostachys japonicus A. Berger extract (EFOJ) and its main constituent compounds. Among all fractions, the highest content of total phenolics was found in EFOJ. The antioxidant activity of EFOJ was confirmed through the 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 1-1-diphenyl-2-picryl-hydrazyl (DPPH), ferric reducing antioxidant power (FRAP) assays and the inhibitory effect of malondialdehyde (MDA). In addition, we ascertained that EFOJ not only decreased the intracellular ROS level, but also protected the neuronal cells against H2O2-induced oxidative stress. In liquid chromatography-mass spectrometry analysis, the following were found to be the main compounds of EFOJ: quercetin-3-O-glucoside, kaempferol-3-O-rutinoside, kaempferol-3-O-glucoside, and kaempferol-3-O-rhamnoside. Consequently, these results suggested that the protective effect on neuronal cells was based on the antioxidant activities of the physiologically active compounds of Orostachys japonicus A. Berger extract, which could therefore help to mitigate neurodegenerative diseases.
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KEYWORD
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antioxidant activity, neurodegenerative disease, neuronal cell protective effect, Orostachys japonicas A. Berger
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